Comparison of Wild Type DNA Sequence of Spike Protein from SARS-CoV-2 with Optimized Sequence on The Induction of Protective Responses Against SARS-Cov-2 Challenge in Mouse Model마우스 모델에서 SARS-CoV-2 공격에 대한 보호 반응 유도에 대한 최적화된 서열과 SARS-CoV-2의 스파이크 단백질 야생형 DNA 서열의 비교Article Published on 2022-12-312022-09-11 Journal: Human vaccines & immunotherapeutics [Category] COVID19(2023년), SARS, 비임상, 진단, [키워드] acid Against Antiviral binding breath CD4 CD8+ cellular immune responses cellular immunity challenge clinical trial contributing to COVID-19 CTL demonstrated DNA DNA vaccine expression full-length spike protein hACE2 humoral humoral and cellular immunity IFN-γ immune immune responses immunogen in vitro induction mice Model mouse Neutralizing antibodies optimization optimizations pGX-9501 platform Protective Protective effects protective response RBD binding response robust SARS-CoV-2 sequence spike Spike protein subsequent T cell T cells the vaccine Transgenic mouse Type virus wild-type wild-type sequence. [DOI] 10.1080/21645515.2021.2016201 PMC 바로가기 [Article Type] Article
Preclinical assessment and randomized Phase I study of CT-P63, a broadly neutralizing antibody targeting severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)Article Published on 2022-12-012022-11-15 Journal: Emerging Microbes & Infections [Category] COVID19(2023년), SARS, 변종, 비임상, 진단, 치료기술, 치료제, [키워드] acute respiratory syndrome Beta Blood caused clinically Concentration coronavirus COVID-19 creatine phosphokinase CT-P63 deaths declining Delta demonstrated detectable dose double-blind evaluated groups healthy volunteer Immunity in vitro in vivo Infection Infectious virus lung monoclonal monoclonal antibody morbidity and mortality mutated variant Neutralizing Neutralizing activity neutralizing antibody neutralizing antibody. omicron pandemic parallel-group participant peaked phase Placebo placebo-controlled Preclinical prevalent Prophylactic Randomized reduced respiratory tract Safe SARS-CoV-2 SARS-CoV-2 variant SARS-CoV-2 variants of concern Serious Adverse Events significantly single ascending dose study drug TEAE titre Tolerability Transgenic mouse treated Treatment treatment-emergent adverse event vaccination variants variants of SARS-CoV-2 wild-type [DOI] 10.1080/22221751.2022.2117094 PMC 바로가기
Identification and characterization of the anti-SARS-CoV-2 activity of cationic amphiphilic steroidal compounds양이온성 양친매성 스테로이드 화합물의 항 SARS-CoV-2 활성의 확인 및 특성화Article Published on 2022-12-012022-09-11 Journal: Virulence [Category] COVID19(2023년), SARS, 변종, 유전자 메커니즘, 치료제, [키워드] aggravation ameliorated anti-SARS-CoV-2 activities Anti-SARS-CoV-2 Activity Antiviral antiviral activity cationic caused cell lines characterization cholesterol complex Compound compounds COVID-19 pandemic COVID-19 patient cytopathic Cytopathic effect Drug discovery effective envelope envelope proteins epiandrosterone followed by hACE2 HEK293 host cell host cells identification in vitro in vivo indicated inhibited initial involved life Lipid mechanism membrane modulate modulation Mortality Pathogenesis progeny protective effect Protective effects Proteins Quality of life reduce reduce COVID-19 aggravation SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 replication selected Selection single mutation single mutations specific treatment spike steroidal compounds. subversion Transgenic mouse translate transmembrane Treatment treatment of COVID-19 patients Vero Vero CCL81 cell Vero CCL81 cells viral replication Viral RNA viral variant Viral variants [DOI] 10.1080/21505594.2022.2085793 PMC 바로가기 [Article Type] Article
A Bacteriophage-Based, Highly Efficacious, Needle- and Adjuvant-Free, Mucosal COVID-19 VaccineArticle Published on 2022-08-302022-11-15 Journal: mBio [Category] COVID19(2023년), SARS, 변종, 진단, [키워드] addition adenovirus-based Administered administration ambient Antibody titer bacteriophage CRISPR engineering bacteriophage T4 mucosal platform BALB/c broad immunity to SARS-CoV-2 variants bronchoalveolar lavage specimen bronchoalveolar lavage specimens caused CD4 CD8 Cell-mediated immune responses cellular immune responses Complete COVID COVID-19 CRISPR cytokine death delta variant dose drug effective elicited enzyme Epidemic exhibited exterior food generate globe gut microbiota hACE2 humoral Immunity induce Infection injected interior intramuscularly intranasal intranasally lung lesion mice mouse-adapted SARS-CoV-2 mRNA mucosal nasal needle needle-free intranasal vaccine not affect nucleocapsid protein omicron other respiratory infection pandemic Pathogens phage T4 platform predicted Prevent provide provided response responses robust SARS-CoV-2 SARS-CoV-2 vaccine SARS-CoV-2 variant SARS-CoV-2 variants secretory IgA sera spike sterilizing immunity. T cell temperature the United State the vaccine Transgenic mouse Transmission trimer Vaccine variants Viral transmission Virus neutralization virus-neutralizing antibody World Health Organization [DOI] 10.1128/mbio.01822-22 PMC 바로가기
SARS-CoV-2 infection impairs the insulin/IGF signaling pathway in the lung, liver, adipose tissue, and pancreatic cells via IRF1SARS-CoV-2 감염은 IRF1을 통해 폐, 간, 지방 조직 및 췌장 세포의 인슐린/IGF 신호 전달 경로를 손상시킵니다Article Published on 2022-08-012022-09-11 Journal: Metabolism: clinical and experimental [Category] COVID19(2023년), SARS, 변종, 유전자 메커니즘, [키워드] Activation adeno-associated virus adipose Adipose tissue AKT ameliorated analyzed association Autopsy Calu3 Calu3 cell CaLu3 cells Cell cell survival cells condition contribute COVID-19 COVID-19 pathology COVID-19 patient COVID-19 patients COVID-19 severity Critical critical patient critical patients cytokine determine Dexamethasone diabetes Diabetic diabetic patients Dihydrotestosterone Endocrine energy metabolism European population evaluate Evidence exacerbated exhibited Gain Gene Expression Genes GFP HEK293T Hospitalized Host human ACE2 human cells hyperglycemia impair impairment infected with SARS-CoV-2 insulin insulin resistance Insulin signaling Insulin/IGF signaling pathway interferon regulatory factor Interferone intron IRF1 liver lung Lungs male Male sex MAPK metabolic metabolic abnormalities Metabolic abnormality metabolism mouse model mTOR Multiorgan dysfunction Multiple organ damage Non-COVID-19 Non-COVID-19 patients obese obesity Older Older age Organoid overexpressing pancreatic Pancreatic cell pancreatic islets pathology patients patients with COVID-19 PI3K potential mechanism Prevalence provide Regulation reported Repository risk factor Risk factors SARS-CoV-2 SARS-CoV-2 infected cell SARS-CoV-2 infected cells SARS-COV-2 infection serotype severe COVID-19 severity Signaling signaling pathway significantly stem cell survival tissue tissue damage Tissue damage. tissues Transcriptome transcriptome dataset transcriptome datasets Transgenic mouse variant Whole blood whole blood transcriptome with COVID-19 [DOI] 10.1016/j.metabol.2022.155236 PMC 바로가기 [Article Type] Article
A third vaccination with a single T cell epitope confers protection in a murine model of SARS-CoV-2 infection단일 T 세포 에피토프를 사용한 세 번째 백신 접종은 SARS-CoV-2 감염의 쥐 모델에서 보호를 제공합니다Article Published on 2022-07-082022-09-12 Journal: Nature Communications [Category] COVID19(2023년), MERS, SARS, 변종, 진단, [키워드] absence approach booster vaccination CD8 characterized cytokine production develop dose dose regimen elicit epitope epitope peptide fraction IMPROVE K18-hACE2 mechanism memory T cells murine model Neutralizing antibodies peptide repeated SARS-CoV-2 SARS-COV-2 infection spike T cell T cell response Transgenic mouse understanding vaccination Vaccine vaccine dose [DOI] 10.1038/s41467-022-31721-6 PMC 바로가기 [Article Type] Article
A mRNA Vaccine Encoding for a RBD 60-mer Nanoparticle Elicits Neutralizing Antibodies and Protective Immunity Against the SARS-CoV-2 Delta Variant in Transgenic K18-hACE2 MiceRBD 60-mer 나노입자를 암호화하는 mRNA 백신은 형질전환 K18-hACE2 마우스에서 SARS-CoV-2 Delta 변이체에 대한 중화 항체 및 보호 면역을 유발합니다Article Published on 2022-07-062022-09-11 Journal: Frontiers in Immunology [Category] COVID19(2023년), MERS, SARS, 변종, 진단, [키워드] Against Antigen COVID-19 pandemic Delta delta variant displaying domain effective elicit encoding flexible hACE2 K18-hACE2 messenger Messenger RNA mRNA mRNA vaccine nanoparticle Neutralizing neutralizing antibody Neutralizing antibody response platform protective immunity provide RBD RBDs Receptor binding domain Receptor-binding domain receptor-binding domain. RNA vaccine robust SARS-CoV-2 SARS-CoV-2 variant severe disease Spread the RBD the SARS-CoV-2 Transgenic mouse Vaccine [DOI] 10.3389/fimmu.2022.912898 PMC 바로가기 [Article Type] Article
K18- and CAG-hACE2 Transgenic Mouse Models and SARS-CoV-2: Implications for Neurodegeneration ResearchK18- 및 CAG-hACE2 형질전환 마우스 모델 및 SARS-CoV-2: 신경변성 연구에 대한 시사점Review Published on 2022-06-282022-09-11 Journal: Molecules [Category] COVID19(2023년), SARS, 유전자 메커니즘, [키워드] absence ACE2 acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 angiotensin Angiotensin-converting enzyme angiotensin-converting enzyme 2 antiviral drug Autopsy Brain brain regions brain tissue CAG-hACE2 transgenic mice caused cells Central nervous system Confusion Consequences coronavirus COVID-19 COVID-19 patient COVID-19 patients Cytokines demonstrated develop direct infection disorder disorders dissect Encephalitis entry receptor enzyme evaluate glial cell global pandemic hACE2 helping human Angiotensin-converting enzyme Human brain increase in infected with SARS-CoV-2 Inflammation K18 K18-transgenic mice leukocyte leukocyte infiltration life manifestation mechanism Meningitis Mental Model molecular mouse Mouse models mucosa neuroinvasion. neurological Neurological complication Neurological complications neuronal neurons olfactory Patient periphery pro-inflammatory cytokine pro-inflammatory cytokines provoke Quality of life reported responses robust SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 neuroinvasion severe acute respiratory syndrome Coronavirus suggested survivor therapy transgenic Transgenic mouse Vaccine development [DOI] 10.3390/molecules27134142 PMC 바로가기 [Article Type] Review
An ADAM17-Neutralizing Antibody Reduces Inflammation and Mortality While Increasing Viral Burden in a COVID-19 Mouse ModelADAM17 중화 항체는 COVID-19 마우스 모델에서 바이러스 부담을 증가시키면서 염증과 사망률을 감소시킵니다Article Published on 2022-06-102022-09-12 Journal: Frontiers in Immunology [Category] COVID19(2023년), MERS, SARS, 진단, [키워드] ACE2 ADAM17 ADAM19 adverse outcome Alter angiotensin anti-viral role antibody appear blockade blocked burden Cell cell entry receptor cellular activation cleave COVID-19 COVID-19 pathogenesis COVID-19 patients Critical ectodomain Increasing Inflammation Inflammatory inflammatory cascade K18-hACE2 less lung Lung pathology lung; metalloproteinase mice Model monoclonal antibody mouse mouse model pathway Proinflammatory cytokine promote protease Proteins SARS-CoV SARS-CoV-2 SARS-CoV-2 viruses significantly increased significantly lower therapeutic TNF-α Transgenic mouse transmembrane viral burden virus virus. while [DOI] 10.3389/fimmu.2022.918881 PMC 바로가기 [Article Type] Article
Pyronaridine Protects against SARS-CoV-2 Infection in Mouse파이로나리딘은 마우스의 SARS-CoV-2 감염으로부터 보호합니다Article Published on 2022-06-102022-09-11 Journal: ACS Infectious Diseases [Category] COVID19(2023년), SARS, 진단, 치료제, [키워드] Antiviral antiviral activity antiviral drug antiviral drugs approved assist Cell cells chemokine coronavirus COVID-19 demonstrated Ebola Efficacy evaluated in vitro in vivo infiltration inhibit SARS-CoV-2 inhibited involved K18-hACE lung Lung pathology Lungs M pro mice molecular mechanism mouse pro-inflammatory pro-inflammatory cytokines pyronaridine reducing reduction in Remdesivir Replication respiratory Respiratory Coronavirus SARS-CoV-2 SARS-CoV-2 replication severe acute respiratory coronavirus Spike protein spike protein. statistically significant reduction structure activity relationship the SARS-CoV-2 therapeutic Transgenic mouse Treatment Viral Viral load [DOI] 10.1021/acsinfecdis.2c00091 PMC 바로가기 [Article Type] Article